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Effect of antisperm antibodies present in human follicular fluid upon the acrosome reaction and sperm-zona pellucida interaction.
Marín-Briggiler, Clara I; Vazquez-Levin, Mónica H; Gonzalez-Echeverría, Fernanda; Blaquier, Jorge A; Miranda, Patricia V; Tezón, Jorge G.
Affiliation
  • Marín-Briggiler CI; Instituto de Biología y Medicina Experimental, CONICET-UBA, Vuelta de Obligado, Buenos Aires, Argentina. cmarin@dna.uba.ar
Am J Reprod Immunol ; 50(3): 209-19, 2003 Sep.
Article in En | MEDLINE | ID: mdl-14629025
PROBLEM: To determine the ability of IgGs isolated from follicular fluids (hFFIgGs) to induce the acrosome reaction (AR) in human spermatozoa and to inhibit sperm-zona pellucida (ZP) interaction. METHOD OF STUDY: Incubation of capacitated spermatozoa with hFFIgGs (n = 40) and assessment of their effect on the AR or hemizona (HZ) assay in a condition that allows sperm-ZP interaction, avoiding acrosomal exocytosis. RESULTS: hFFIgGs from different women varied in their ability of inducing the AR. Those hFFIgGs with the highest AR-inducing capacity evoked the exocytotic response in most of the different sperm donors tested [high Induction Frequency (IF)]. Some of these antibodies were also able of inhibiting sperm binding to ZP [low HZ Index (HZI)]. A significant correlation was found between the IF and the HZI for each hFFIgG. CONCLUSIONS: Human follicular fluid contains antibodies capable of inducing the AR and inhibiting sperm-ZP binding, suggesting that they could be directed towards ZP receptors. hFFIgGs would constitute a tool for the identification of sperm entities involved in fertilization.
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Collection: 01-internacional Database: MEDLINE Main subject: Spermatozoa / Zona Pellucida / Immunoglobulin G / Follicular Fluid / Acrosome Reaction Limits: Adult / Female / Humans / Male Language: En Journal: Am J Reprod Immunol Year: 2003 Document type: Article Affiliation country: Argentina Country of publication: Denmark
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Collection: 01-internacional Database: MEDLINE Main subject: Spermatozoa / Zona Pellucida / Immunoglobulin G / Follicular Fluid / Acrosome Reaction Limits: Adult / Female / Humans / Male Language: En Journal: Am J Reprod Immunol Year: 2003 Document type: Article Affiliation country: Argentina Country of publication: Denmark