Tetrahedral intermediates in thiamin diphosphate-dependent decarboxylations exist as a 1',4'-imino tautomeric form of the coenzyme, unlike the michaelis complex or the free coenzyme.

Nemeria, Natalia; Baykal, Ahmet; Joseph, Ebenezer; Zhang, Sheng; Yan, Yan; Furey, William; Jordan, Frank

Nemeria, Natalia; Baykal, Ahmet; Joseph, Ebenezer; Zhang, Sheng; Yan, Yan; Furey, William; Jordan, Frank.

Affiliation

Nemeria N; Department of Chemistry at Rutgers, the State University, Newark, New Jersey 07102, USA. nemeria@andromeda.rutgers.edu

Biochemistry ; 43(21): 6565-75, 2004 Jun 01.

Article in En | MEDLINE | ID: mdl-15157089

Subject(s)

Coenzymes/chemistry; Coenzymes/metabolism; Phosphonoacetic Acid/analogs & derivatives; Thiamine Pyrophosphate/chemistry; Binding Sites; Catalysis; Circular Dichroism; Decarboxylation; Fungal Proteins/chemistry; Fungal Proteins/metabolism; Models, Chemical; Models, Molecular; Phosphonoacetic Acid/chemistry; Point Mutation; Protein Conformation; Pyruvate Decarboxylase/chemistry; Pyruvate Decarboxylase/metabolism; Pyruvate Dehydrogenase Complex/chemistry; Pyruvate Dehydrogenase Complex/genetics; Pyruvate Dehydrogenase Complex/metabolism; Thiamine Pyrophosphate/metabolism; Titrimetry

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Collection: 01-internacional Database: MEDLINE Main subject: Phosphonoacetic Acid / Thiamine Pyrophosphate / Coenzymes Language: En Journal: Biochemistry Year: 2004 Document type: Article Affiliation country: United States Country of publication: United States

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