Rapid sexing of bovine preimplantation embryos using loop-mediated isothermal amplification.

Hirayama, Hiroki; Kageyama, Soichi; Moriyasu, Satoru; Sawai, Ken; Onoe, Sadao; Takahashi, Yoshiyuki; Katagiri, Seiji; Toen, Keiko; Watanabe, Keiko; Notomi, Tsugunori; Yamashina, Hidenari; Matsuzaki, Sigenori; Minamihashi, Akira

Hirayama, Hiroki; Kageyama, Soichi; Moriyasu, Satoru; Sawai, Ken; Onoe, Sadao; Takahashi, Yoshiyuki; Katagiri, Seiji; Toen, Keiko; Watanabe, Keiko; Notomi, Tsugunori; Yamashina, Hidenari; Matsuzaki, Sigenori; Minamihashi, Akira.

Affiliation

Hirayama H; Hokkaido Animal Research Center, Shintoku, 081-0038, Japan. h.hirayama@agri.pref.hokkaido.jp

Theriogenology ; 62(5): 887-96, 2004 Sep 01.

Article in En | MEDLINE | ID: mdl-15251240

Subject(s)

Cattle; Embryo, Mammalian; Embryonic Development; Nucleic Acid Amplification Techniques/veterinary; Sex Determination Analysis/veterinary; Animals; Blastomeres/chemistry; DNA/isolation & purification; Embryo Transfer/veterinary; Endopeptidase K; Female; Hot Temperature; Polymerase Chain Reaction; Polysorbates; Pregnancy; Sensitivity and Specificity; Sex Determination Analysis/methods; Sodium Hydroxide; Time Factors

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Collection: 01-internacional Database: MEDLINE Main subject: Sex Determination Analysis / Cattle / Nucleic Acid Amplification Techniques / Embryonic Development / Embryo, Mammalian Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals / Pregnancy Language: En Journal: Theriogenology Year: 2004 Document type: Article Affiliation country: Japan Country of publication: United States

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