Recombinant expression, purification, and kinetic and inhibitor characterisation of human site-1-protease.

Bodvard, Kristofer; Mohlin, Johanna; Knecht, Wolfgang

Bodvard, Kristofer; Mohlin, Johanna; Knecht, Wolfgang.

Affiliation

Bodvard K; Molecular Pharmacology - Target Production, AstraZeneca R&D Mölndal, 431 83 Mölndal, Sweden.

Protein Expr Purif ; 51(2): 308-19, 2007 Feb.

Article in En | MEDLINE | ID: mdl-16973377

Subject(s)

Proprotein Convertases/genetics; Serine Endopeptidases/genetics; Amino Acid Sequence; Animals; Buffers; Capsid Proteins/metabolism; Cations, Divalent/pharmacology; Edetic Acid/pharmacology; Egtazic Acid/pharmacology; Electrophoresis, Polyacrylamide Gel; Glycosylation; Humans; Kinetics; Lassa virus; Molecular Sequence Data; Proprotein Convertases/antagonists & inhibitors; Proprotein Convertases/isolation & purification; Proprotein Convertases/metabolism; Recombinant Proteins/antagonists & inhibitors; Recombinant Proteins/biosynthesis; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Sequence Alignment; Serine Endopeptidases/isolation & purification; Serine Endopeptidases/metabolism; Spodoptera; Substrate Specificity

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Collection: 01-internacional Database: MEDLINE Main subject: Serine Endopeptidases / Proprotein Convertases Limits: Animals / Humans Language: En Journal: Protein Expr Purif Journal subject: BIOLOGIA MOLECULAR Year: 2007 Document type: Article Affiliation country: Sweden

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