Effect of granulocyte-macrophage colony stimulating factor on growth, resistance to freezing and thawing and re-expansion of murine blastocysts.
Reprod Biomed Online
; 14(1): 96-101, 2007 Jan.
Article
in En
| MEDLINE
| ID: mdl-17207341
Two-cell murine embryos were cultured for 72 h in the presence or absence of granulocyte-macrophage colony stimulating factor (GM-CSF), frozen for 60 days and, after thawing, cultured for an additional 24 h in the presence or absence of GM-CSF. During the initial 72 h period, GM-CSF did not influence the percentage of embryos reaching the expanded blastocyst stage, but there was a significant increase (P < 0.05) in the number of cells in the embryos grown with GM-CSF. Survival after thawing was not affected by previous exposure to GM-CSF, but re-expansion of the blastocoele was diminished in that group. Exposure to GM-CSF during the post-thaw period greatly enhanced re-expansion of the blastocoele. The presence of human serum albumin in the culture media is thought to have masked the beneficial effect of GM-CSF upon embryos.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blastocyst
/
Cryopreservation
/
Granulocyte-Macrophage Colony-Stimulating Factor
/
Embryonic Development
Limits:
Animals
/
Female
/
Humans
Language:
En
Journal:
Reprod Biomed Online
Journal subject:
MEDICINA REPRODUTIVA
Year:
2007
Document type:
Article
Affiliation country:
Argentina
Country of publication:
Netherlands