Cytophotometric assessment of Feulgen-DNA and coomassie-total cell protein in adrenocortical fasciculata cells of noise-exposed Wistar rats.
Cell Biochem Funct
; 9(4): 287-92, 1991 Oct.
Article
in En
| MEDLINE
| ID: mdl-1725508
ABSTRACT
The present investigation was undertaken to examine possible noise-induced alterations in adrenal fasciculata cell (AFC) metabolism, and also to determine if the magnitude of these changes differs in male versus female rats. Wistar rats approximately 3 months old were exposed to intense noise for 60 min (100 dB, re 2 x 10(-5) N(m2)-1, 350-20,000 Hz); control rats were housed under identical conditions, at an ambient noise level of 40-60 dB. Adrenal fasciculata cells (AFC) from each animal were examined for noise-induced alterations in Feulgen-DNA reactivity (as an indicator of chromatin template activity) and Coomassie-total cell protein levels using scanning-integrating cytophotometry. The results provide evidence that intense noise elicited a marked AFC metabolic enhancement in both male and female rats; the degree of this enhancement was more pronounced in males. This disparity may be due to pre-existing differences in male versus female AFC enzymatic capability and subsequent responsiveness to noise-induced activation.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cytophotometry
/
DNA
/
Zona Fasciculata
/
Proteins
/
Noise
Limits:
Animals
Language:
En
Journal:
Cell Biochem Funct
Year:
1991
Document type:
Article