Development and validation of an RNA- and DNA-based quantitative PCR assay for determination of Kudoa thyrsites infection levels in Atlantic salmon Salmo salar.

Funk, Valerie A; Raap, Monique; Sojonky, Ken; Jones, Simon; Robinson, John; Falkenberg, Christy; Miller, Kristina M

Funk, Valerie A; Raap, Monique; Sojonky, Ken; Jones, Simon; Robinson, John; Falkenberg, Christy; Miller, Kristina M.

Affiliation

Funk VA; BC Centre for Aquatic Health Sciences, 621 Island Highway, PO Box 277, Campbell River, British Columbia V9W 5B1, Canada. val.funk@cahs-bc.ca

Dis Aquat Organ ; 75(3): 239-49, 2007 May 09.

Article in En | MEDLINE | ID: mdl-17629119

Subject(s)

Eukaryota/isolation & purification; Fish Diseases/diagnosis; Polymerase Chain Reaction/veterinary; Protozoan Infections, Animal/diagnosis; Salmo salar/parasitology; Animals; Cathepsin L; Cathepsins/analysis; Cathepsins/genetics; Cysteine Endopeptidases/analysis; Cysteine Endopeptidases/genetics; DNA/analysis; DNA Primers/chemistry; Eukaryota/genetics; Fish Diseases/parasitology; Logistic Models; Meat/standards; Muscles/parasitology; Polymerase Chain Reaction/methods; Protozoan Infections, Animal/parasitology; RNA, Messenger/analysis; RNA, Ribosomal, 18S/analysis; RNA, Ribosomal, 18S/genetics; Reproducibility of Results; Sensitivity and Specificity

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Collection: 01-internacional Database: MEDLINE Main subject: Protozoan Infections, Animal / Polymerase Chain Reaction / Salmo salar / Eukaryota / Fish Diseases Type of study: Diagnostic_studies / Prognostic_studies / Risk_factors_studies Limits: Animals Language: En Journal: Dis Aquat Organ Journal subject: BIOLOGIA / MEDICINA VETERINARIA / MICROBIOLOGIA Year: 2007 Document type: Article Affiliation country: Canada Country of publication: Germany

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