Production and secretion of a recombinant Vibrio parahaemolyticus chitinase by Escherichia coli and its purification from the culture medium.

Kadokura, Kazunari; Sakamoto, Yusuke; Saito, Kaori; Ikegami, Takanori; Hirano, Takako; Hakamata, Wataru; Oku, Tadatake; Nishio, Toshiyuki

Kadokura, Kazunari; Sakamoto, Yusuke; Saito, Kaori; Ikegami, Takanori; Hirano, Takako; Hakamata, Wataru; Oku, Tadatake; Nishio, Toshiyuki.

Affiliation

Kadokura K; Department of Biological Chemistry, College of Bioresource Sciences, Nihon University, Fujisawa, Kanagawa 252-8510, Japan.

Biosci Biotechnol Biochem ; 71(11): 2848-51, 2007 Nov.

Article in En | MEDLINE | ID: mdl-17986788

Subject(s)

Bacterial Proteins/biosynthesis; Bacterial Proteins/isolation & purification; Chitinases/biosynthesis; Chitinases/isolation & purification; Recombinant Proteins/biosynthesis; Recombinant Proteins/isolation & purification; Vibrio parahaemolyticus/enzymology; Bacterial Proteins/genetics; Chitinases/genetics; Cloning, Molecular; Culture Media/chemistry; Escherichia coli/genetics; Genome, Bacterial; Open Reading Frames/genetics; Protein Sorting Signals/genetics; Recombinant Proteins/metabolism; Transformation, Genetic; Vibrio parahaemolyticus/genetics

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Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Vibrio parahaemolyticus / Recombinant Proteins / Chitinases Language: En Journal: Biosci Biotechnol Biochem Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2007 Document type: Article Affiliation country: Japan Country of publication: United kingdom

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