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Identification of vibrio cholerae and vibrio mimicus by multilocus sequence analysis (MLSA).
Thompson, Cristiane C; Thompson, Fabiano L; Vicente, Ana Carolina P.
Affiliation
  • Thompson CC; Instituto Oswaldo Cruz, Rio de Janeiro, Brazil. thompson@ioc.fiocruz.br
Int J Syst Evol Microbiol ; 58(Pt 3): 617-21, 2008 Mar.
Article in En | MEDLINE | ID: mdl-18319466
Vibrio cholerae and Vibrio mimicus have similar phenotypes and genomes making rapid differentiation of these two species difficult. The first standard multilocus sequence analysis (MLSA) scheme for the identification of these species is described. A collection of 45 representative isolates from different geographical regions and hosts was examined using segments of the housekeeping genes pyrH, recA and rpoA. Overall, the closest phylogenetic neighbours of these species were Vibrio furnissii and Vibrio fluvialis. V. cholerae and V. mimicus formed separate species clusters on the basis of each gene, suggesting that these genes are useful as identification markers. These species clusters arose by the accumulation of point mutations. The pyrH gene showed the highest resolution for differentiating V. cholerae and V. mimicus. The maximum interspecies pyrH gene sequence similarity was 91 %. Clearly, V. mimicus strains were more heterogeneous than V. cholerae strains at the three loci. It is suggested that vibrio species may be defined on the basis of MLSA data. A vibrio species was defined as a group of strains forming a monophyletic group on the basis of these loci and with an intraspecific sequence similarity of at least 95 %. V. cholerae and V. mimicus isolates can be readily identified through the open database resource 'The Taxonomy of Vibrios' (http://www.taxvibrio.lncc.br/).
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rec A Recombinases / Vibrio cholerae / DNA-Directed RNA Polymerases / Sequence Analysis, DNA / Nucleoside-Phosphate Kinase / Vibrio mimicus Type of study: Diagnostic_studies / Evaluation_studies Limits: Humans Language: En Journal: Int J Syst Evol Microbiol Journal subject: MICROBIOLOGIA Year: 2008 Document type: Article Affiliation country: Brazil Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Rec A Recombinases / Vibrio cholerae / DNA-Directed RNA Polymerases / Sequence Analysis, DNA / Nucleoside-Phosphate Kinase / Vibrio mimicus Type of study: Diagnostic_studies / Evaluation_studies Limits: Humans Language: En Journal: Int J Syst Evol Microbiol Journal subject: MICROBIOLOGIA Year: 2008 Document type: Article Affiliation country: Brazil Country of publication: United kingdom