Acute stretch promotes endothelial cell proliferation in wounded healing mouse skin.
Arch Dermatol Res
; 300(9): 495-504, 2008 Oct.
Article
in En
| MEDLINE
| ID: mdl-18330587
ABSTRACT
We have developed a novel in vivo model utilizing acute stretch to investigate endothelial cell proliferation as a marker of vascular growth in healing mouse skin. This study is a follow-up to ones revealing immediate stretch improves blood flow, decreases total tissue necrosis, and induces tissue insulin transcription. Dorsal distally based flaps of skin were stretched for 3 min using linear (skin hook) plus hemispherical load cycling (inflated subcutaneous silicone catheter). Unstretched, wounded skin along the back and sternum served as postoperative controls. Laser Doppler flowmetry demonstrated a threefold increase in flap perfusion at postoperative day 7. A stretch-induced sixfold increase in endothelial cell mitogenesis accompanied enhancements in blood flow and extracorporal wound healing over the sternum. Western blots revealed up-regulation/activation of insulin and mitogenic signaling intermediates in stretched skin. Activated insulin and insulin growth factor receptors (pIR/pIGFR), protein kinase B (Akt, pAkt), vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor 2 (flk-1) were among the identified stretch-responsive intermediates. These results indicate the benefits of acute stretch are mediated through enhanced vascularity as evidenced by endothelial cell mitogenesis and up-regulation/activation of insulin and key angiogenic effectors in dorsal distally based skin flaps.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Skin
/
Wound Healing
/
Endothelium, Vascular
/
Cell Proliferation
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Arch Dermatol Res
Year:
2008
Document type:
Article
Affiliation country:
United States