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B-1 cells facilitate Paracoccidioides brasiliensis infection in mice via IL-10 secretion.
Popi, Ana Flavia; Godoy, Luiz Claudio; Xander, Patricia; Lopes, Jose Daniel; Mariano, Mario.
Affiliation
  • Popi AF; Department of Microbiology, Immunology and Parasitology, Universidade Federal de São Paulo, Rua Botucatu 862, São Paulo, SP, Brazil.
Microbes Infect ; 10(7): 817-24, 2008 Jun.
Article in En | MEDLINE | ID: mdl-18555722
Protective immunity in paracoccidioidomycosis is mainly mediated by cellular immunity. The role of B cells in this disease, in particular B-1 cells, is poorly understood. The aim of this study was to characterize the participation of B-1 cells in resistance or susceptibility of BALB/c and BALB/Xid mice to P. brasiliensis (Pb) pulmonary infection. BALB/Xid, which lacks B-1 cells, exhibited higher resistance to infection when compared with BALB/c mice. However, adoptive transfer of B-1 cells to BALB/Xid mice drastically increased the susceptibility of these animals to Pb infection. The fungal burden in BALB/c and B-1-reconstituted BALB/Xid was significantly higher as compared to BALB/Xid strain. Compact, well-organized granulomas were observed in the lungs of BALB/Xid mice, whereas large lesions with necrotic center with a plethora of fungi developed in BALB/c mice. It was also shown that B-1 cells impair phagocytosis of Pb by macrophages in vitro via secretion of IL-10, which was increased upon stimulation with a purified Pb antigen, gp43. Finally, in vivo blockade of IL-10 led to a better control of infection by the highly susceptible B10.A mouse. These findings suggest that B-1 cells play a major role in resistance/susceptibility to Pb infection in murine models, most likely via production of IL-10.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Paracoccidioides / Paracoccidioidomycosis / B-Lymphocytes / Interleukin-10 Type of study: Prognostic_studies Limits: Animals Country/Region as subject: America do sul / Brasil Language: En Journal: Microbes Infect Journal subject: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA Year: 2008 Document type: Article Affiliation country: Brazil Country of publication: France

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Paracoccidioides / Paracoccidioidomycosis / B-Lymphocytes / Interleukin-10 Type of study: Prognostic_studies Limits: Animals Country/Region as subject: America do sul / Brasil Language: En Journal: Microbes Infect Journal subject: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA Year: 2008 Document type: Article Affiliation country: Brazil Country of publication: France