Detection of antigens in urine of patients with acute falciparum and vivax malaria infections.
Am J Trop Med Hyg
; 45(4): 453-62, 1991 Oct.
Article
in En
| MEDLINE
| ID: mdl-1951853
Using an antigen-capture, dot-blot assay, antigens were detected in the urine of 50 patients infected with Plasmodium falciparum. Antigens were also detected in 12/15 patients who had no detectable parasitemias 1-2 weeks after chemotherapy. By Western blotting and immunoprecipitation, four predominant antigens were identified with the following molecular masses (Mr) and isoelectric points (pI): antigen 1, 200 kDa, pI 6.4-6.27; antigen 2, 180 kDa, pI 5.2-4.8; antigen 3, 150 kDa, pI 5.5; antigen 4, 96 kDa, pI 5.1-4.8. These antigens were heat stable to 100 degrees C for 5 min. Antigens were also detected in the urine of 35 patients with acute P. vivax infections by Western blotting and dot-blot analysis and 10/10 patients three weeks following chemotherapy. The antigens had Mr of 200, 170, and 130 kDa.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plasmodium falciparum
/
Plasmodium vivax
/
Malaria, Vivax
/
Malaria, Falciparum
/
Antigens, Protozoan
Type of study:
Diagnostic_studies
Limits:
Adolescent
/
Adult
/
Animals
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Am J Trop Med Hyg
Year:
1991
Document type:
Article
Affiliation country:
Brazil
Country of publication:
United States