Expressed protein ligation for metalloprotein design and engineering.

Clark, Kevin M; van der Donk, Wilfred A; Lu, Yi

Clark, Kevin M; van der Donk, Wilfred A; Lu, Yi.

Affiliation

Clark KM; Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.

Methods Enzymol ; 462: 97-115, 2009.

Article in En | MEDLINE | ID: mdl-19632471

Subject(s)

Azurin/chemistry; Bacterial Proteins/chemistry; Metalloproteins/chemistry; Peptides/chemical synthesis; Protein Engineering/methods; Amino Acid Sequence; Amino Acid Substitution; Amino Acids/physiology; Binding Sites; Copper/metabolism; Cysteine/chemistry; Electron Spin Resonance Spectroscopy; Inteins; Ligands; Methionine/analogs & derivatives; Methionine/chemistry; Molecular Sequence Data; Protein Splicing; Pseudomonas aeruginosa/enzymology; Recombinant Fusion Proteins/biosynthesis; Selenocysteine/analogs & derivatives; Selenocysteine/chemistry; Spectrophotometry

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptides / Azurin / Bacterial Proteins / Protein Engineering / Metalloproteins Language: En Journal: Methods Enzymol Year: 2009 Document type: Article Affiliation country: United States

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