Selection of buffer pH by the isoelectric point of the antigen for the efficient heat-induced epitope retrieval: re-appraisal for nuclear protein pathobiology.

Kajiya, Hanako; Takekoshi, Susumu; Takei, Mao; Egashira, Noboru; Miyakoshi, Takashi; Serizawa, Akihito; Teramoto, Akira; Osamura, Robert Y

Kajiya, Hanako; Takekoshi, Susumu; Takei, Mao; Egashira, Noboru; Miyakoshi, Takashi; Serizawa, Akihito; Teramoto, Akira; Osamura, Robert Y.

Affiliation

Kajiya H; Department of Pathology, Tokai University School of Medicine, 143 Shimokasuya, Boseidai Isehara, Kanagawa, 259-1193, Japan. hanakokj@is.icc.u-tokai.ac.jp

Histochem Cell Biol ; 132(6): 659-67, 2009 Dec.

Article in En | MEDLINE | ID: mdl-19768463

Subject(s)

Epitopes/analysis; Immunohistochemistry/methods; Antibodies; Antigens; Buffers; Formaldehyde; Hot Temperature; Humans; Hydrogen-Ion Concentration; Immunohistochemistry/standards; Isoelectric Point; Nuclear Proteins/analysis; Nuclear Proteins/immunology; Paraffin Embedding; Tissue Fixation

Fulltext

Add to My VHL

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Immunohistochemistry / Epitopes Limits: Humans Language: En Journal: Histochem Cell Biol Journal subject: CITOLOGIA / HISTOCITOQUIMICA Year: 2009 Document type: Article Affiliation country: Japan Country of publication: Germany

Fulltext

Add to My VHL

XML

PubMed Links

Search on Google