Determination of polyamines in organic extracts from roots of Canavalia ensiformis by capillary electrophoresis.
J Environ Sci Health B
; 45(4): 325-9, 2010 May.
Article
in En
| MEDLINE
| ID: mdl-20401784
A rapid, selective and specific capillary zone electrophoresis method to determine polyamines in organic extracts from roots of Canavalia ensiformis (Jack Beans) was developed using ultra violet (UV) detection. Canavalia ensiformis is relatively free from diseases and it is used as reference in allelopathy studies. Polyamines are widely distributed in plant and it could be involved in plant pathogen interactions. Optimal separation was achieved using 15 mmol L(-1)formic acid (pH 3.0) + 4 mmol L(-1) imidazole as a background electrolyte. It was possible to identify and quantify the polyamines on herbal samples in the presence of other phytochemical substances and analyze them quickly (up to 6 min). The applicability of this method was evaluated in crude organic extracts from roots of Canavalia ensiformis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Organic Chemicals
/
Polyamines
/
Plant Roots
/
Electrophoresis, Capillary
/
Canavalia
Type of study:
Prognostic_studies
Language:
En
Journal:
J Environ Sci Health B
Year:
2010
Document type:
Article
Affiliation country:
Brazil
Country of publication:
United kingdom