Association of ATP1A1 gene polymorphism with heat tolerance traits in dairy cattle.
Genet Mol Res
; 9(2): 891-6, 2010 May 11.
Article
in En
| MEDLINE
| ID: mdl-20467982
Heat stress produces oxidative stress and affects the alternation of plasma K(+) and Na(+). Since Na(+),K(+)-ATPase is sensitive to oxidative stress and critical for maintaining the homeostasis of these two ions, we examined the genetic polymorphism of the ATP1A1 gene in 160 Holstein cows using polymerase chain reaction low ionic strength single-strand conformation polymorphism and DNA sequencing methods. G to A at position -14103 in exon 14 and C to T at position -14242 in intron 14 of the bovine ATP1A1 gene were identified, but the former single nucleotide polymorphism was silent with respect to the amino acid sequence of the protein. However, we found significant correlations between ATP1A1 gene polymorphism and the coefficient of heat tolerance (P<0.01) and with respiratory rate (P<0.01). Genotype AC was the most favorable genotype for heat tolerance. This polymorphism site has potential as a genetic marker for heat tolerance traits in dairy cattle breeding.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polymorphism, Genetic
/
Sodium-Potassium-Exchanging ATPase
Type of study:
Risk_factors_studies
Limits:
Animals
Language:
En
Journal:
Genet Mol Res
Journal subject:
BIOLOGIA MOLECULAR
/
GENETICA
Year:
2010
Document type:
Article
Affiliation country:
China
Country of publication:
Brazil