Expression of soluble, active fragments of the morphogenetic protein SpoIIE from Bacillus subtilis using a library-based construct screen.

Rawlings, Andrea E; Levdikov, Vladimir M; Blagova, Elena; Colledge, Vicki L; Mas, Philippe J; Tunaley, James; Vavrova, Ludmila; Wilson, Keith S; Barak, Imrich; Hart, Darren J; Wilkinson, Anthony J

Rawlings, Andrea E; Levdikov, Vladimir M; Blagova, Elena; Colledge, Vicki L; Mas, Philippe J; Tunaley, James; Vavrova, Ludmila; Wilson, Keith S; Barak, Imrich; Hart, Darren J; Wilkinson, Anthony J.

Affiliation

Rawlings AE; Structural Biology Laboratory, Department of Chemistry, University of York, York, UK.

Protein Eng Des Sel ; 23(11): 817-25, 2010 Nov.

Article in En | MEDLINE | ID: mdl-20817757

Subject(s)

Bacillus subtilis/genetics; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Cloning, Molecular/methods; Bacillus subtilis/chemistry; Bacillus subtilis/metabolism; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Cytoskeletal Proteins/metabolism; Gene Expression; Mutagenesis; Protein Binding; Protein Structure, Quaternary; Protein Structure, Tertiary; Solubility

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacillus subtilis / Bacterial Proteins / Cloning, Molecular Language: En Journal: Protein Eng Des Sel Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2010 Document type: Article Affiliation country: United kingdom Country of publication: United kingdom

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