Improved expression and characterization of recombinant human Golgi &#945;1,2-mannosidase I isoforms (IA2 and IC) by Escherichia coli.

Isoyama-Tanaka, Junko; Dohi, Koji; Misaki, Ryo; Fujiyama, Kazuhito

Improved expression and characterization of recombinant human Golgi α1,2-mannosidase I isoforms (IA2 and IC) by Escherichia coli.

Isoyama-Tanaka, Junko; Dohi, Koji; Misaki, Ryo; Fujiyama, Kazuhito.

Affiliation

Isoyama-Tanaka J; International Center for Biotechnology, Osaka University, 21 Yamada-oka, Suita, Osaka 5650871, Japan.

J Biosci Bioeng ; 112(1): 14-9, 2011 Jul.

Article in En | MEDLINE | ID: mdl-21450520

Subject(s)

Escherichia coli/genetics; Golgi Apparatus/enzymology; Mannosidases/genetics; Calcium/metabolism; Cloning, Molecular; Escherichia coli/metabolism; Golgi Apparatus/metabolism; Humans; Hydrogen-Ion Concentration; Mannosidases/metabolism; Protein Isoforms/genetics; Protein Isoforms/metabolism; Substrate Specificity; Temperature

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Escherichia coli / Golgi Apparatus / Mannosidases Limits: Humans Language: En Journal: J Biosci Bioeng Journal subject: ENGENHARIA BIOMEDICA / MICROBIOLOGIA Year: 2011 Document type: Article Affiliation country: Japan Country of publication: Japan

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