Improved technique for reconstituting incredibly high and soluble amounts of tetrameric Kâº channel in natural membranes.

Raja, Mobeen; Vales, Elisabeth

Improved technique for reconstituting incredibly high and soluble amounts of tetrameric Kâº channel in natural membranes.

Raja, Mobeen; Vales, Elisabeth.

Affiliation

Raja M; Institute for Biophysics, Johannes Kepler University Linz, 4040 Linz, Austria. mraja@pmcol.ualberta.ca

J Membr Biol ; 241(3): 141-4, 2011 Jun.

Article in En | MEDLINE | ID: mdl-21573757

Subject(s)

Bacterial Proteins/isolation & purification; Bioengineering/methods; Cell Membrane/metabolism; Escherichia coli/metabolism; Potassium Channels/isolation & purification; Proteolipids/metabolism; Recombinant Proteins/isolation & purification; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cell Membrane/chemistry; Cholic Acids/chemistry; Cloning, Molecular; Detergents/chemistry; Electrophoresis, Polyacrylamide Gel; Escherichia coli/chemistry; Escherichia coli/genetics; Glucosides/chemistry; Plasmids; Potassium Channels/genetics; Potassium Channels/metabolism; Protein Multimerization; Proteolipids/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Solubility

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Proteolipids / Bacterial Proteins / Recombinant Proteins / Potassium Channels / Cell Membrane / Escherichia coli / Bioengineering Language: En Journal: J Membr Biol Year: 2011 Document type: Article Affiliation country: Austria Country of publication: United States

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