Transient transfection of serum-free suspension HEK 293 cell culture for efficient production of human rFVIII.

Swiech, Kamilla; Kamen, Amine; Ansorge, Sven; Durocher, Yves; Picanço-Castro, Virgínia; Russo-Carbolante, Elisa M S; Neto, Mário S A; Covas, Dimas T

Swiech, Kamilla; Kamen, Amine; Ansorge, Sven; Durocher, Yves; Picanço-Castro, Virgínia; Russo-Carbolante, Elisa M S; Neto, Mário S A; Covas, Dimas T.

Affiliation

Swiech K; Regional Blood Center of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil. kamilla@hemocentro.fmrp.usp.br

BMC Biotechnol ; 11: 114, 2011 Nov 24.

Article in En | MEDLINE | ID: mdl-22115125

Subject(s)

Biotechnology/methods; Cell Culture Techniques/methods; Culture Media, Serum-Free; Factor VIII/biosynthesis; Recombinant Proteins/biosynthesis; Transfection/methods; Ammonia/analysis; Blood Cell Count/methods; Enzyme-Linked Immunosorbent Assay; Erythrosine; Glucose/analysis; HEK293 Cells; Humans; Lactic Acid/analysis

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biotechnology / Recombinant Proteins / Factor VIII / Transfection / Culture Media, Serum-Free / Cell Culture Techniques Limits: Humans Language: En Journal: BMC Biotechnol Journal subject: BIOTECNOLOGIA Year: 2011 Document type: Article Affiliation country: Brazil Country of publication: United kingdom

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