Fusing two cytochromes b of Rhodobacter capsulatus cytochrome bc1 using various linkers defines a set of protein templates for asymmetric mutagenesis.

Czapla, Monika; Borek, Arkadiusz; Sarewicz, Marcin; Osyczka, Artur

Czapla, Monika; Borek, Arkadiusz; Sarewicz, Marcin; Osyczka, Artur.

Affiliation

Czapla M; Department of Molecular Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, 30-387 Kraków, Poland.

Protein Eng Des Sel ; 25(1): 15-25, 2012 Jan.

Article in En | MEDLINE | ID: mdl-22119789

Subject(s)

Bacterial Proteins/metabolism; Cytochromes b/metabolism; Protein Engineering/methods; Rhodobacter capsulatus/metabolism; Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Blotting, Western; Cytochromes b/chemistry; Cytochromes b/genetics; Electron Transport Complex III/chemistry; Electron Transport Complex III/genetics; Electron Transport Complex III/metabolism; Models, Molecular; Molecular Sequence Data; Mutagenesis; Protein Multimerization; Protein Structure, Quaternary; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Rhodobacter capsulatus/genetics; Spectrophotometry

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Protein Engineering / Rhodobacter capsulatus / Cytochromes b Language: En Journal: Protein Eng Des Sel Journal subject: BIOQUIMICA / BIOTECNOLOGIA Year: 2012 Document type: Article Affiliation country: Poland Country of publication: United kingdom

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