Antibody-directed lentiviral gene transduction for live-cell monitoring and selection of human iPS and hES cells.

Wu, Dai-tze; Seita, Yasunari; Zhang, Xia; Lu, Chi-Wei; Roth, Monica J

Wu, Dai-tze; Seita, Yasunari; Zhang, Xia; Lu, Chi-Wei; Roth, Monica J.

Affiliation

Wu DT; Department of Pharmacology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey, United States of America.

PLoS One ; 7(4): e34778, 2012.

Article in En | MEDLINE | ID: mdl-22536330

Subject(s)

Embryonic Stem Cells/cytology; Immunoconjugates/genetics; Induced Pluripotent Stem Cells/cytology; Lentivirus/genetics; Antigens, Surface/immunology; Antigens, Surface/metabolism; Cell Differentiation; Cell Separation/methods; Cells, Cultured; Embryoid Bodies/cytology; Embryoid Bodies/metabolism; Embryonic Stem Cells/metabolism; Embryonic Stem Cells/physiology; Flow Cytometry; Genetic Vectors; Green Fluorescent Proteins/biosynthesis; Humans; Immunoconjugates/metabolism; Induced Pluripotent Stem Cells/metabolism; Induced Pluripotent Stem Cells/physiology; Lentivirus/metabolism; Recombinant Proteins/biosynthesis; Transduction, Genetic; Transgenes; Virion/genetics; Virus Internalization

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Lentivirus / Immunoconjugates / Embryonic Stem Cells / Induced Pluripotent Stem Cells Limits: Humans Language: En Journal: PLoS One Journal subject: CIENCIA / MEDICINA Year: 2012 Document type: Article Affiliation country: United States

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