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Architecture of bacterial lipid A in solution. A neutron small-angle scattering study.
Labischinski, H; Vorgel, E; Uebach, W; May, R P; Bradaczek, H.
Affiliation
  • Labischinski H; Robert Koch-Institut des Bundesgesundheitsamtes, Berlin.
Eur J Biochem ; 190(2): 359-63, 1990 Jun 20.
Article in En | MEDLINE | ID: mdl-2364950
The phase structure of isolated bacterial lipid A, the lipid anchor of the lipopolysaccharides of the outer membrane of Gram-negative bacteria, has been investigated by neutron small-angle scattering. The shape of the scattering curves obtained at different H2O/2H2O ratios revealed a lamellar organisation of the lipid A at neutral pH both above and below its main phase temperature (approximately 40-45 degrees C). Analysis of the scattering curves and interpretation of the corresponding thickness distance distribution functions of the lamellar aggregates led to a model in which the lipid A molecules form a bilayer of about 5 nm in thickness. This value for the thickness of the bilayer, as well as the neutron-scattering density profile across the bilayer, can be explained by a molecular model which shows interdigitation of the fatty acid chains of the lipid A.
Subject(s)
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Collection: 01-internacional Database: MEDLINE Main subject: Salmonella / Lipid A Language: En Journal: Eur J Biochem Year: 1990 Document type: Article Country of publication: United kingdom
Search on Google
Collection: 01-internacional Database: MEDLINE Main subject: Salmonella / Lipid A Language: En Journal: Eur J Biochem Year: 1990 Document type: Article Country of publication: United kingdom