Ultrastructure of phagocytosed Paracoccidioides brasiliensis in nonactivated or activated macrophages.
Infect Immun
; 58(8): 2628-36, 1990 Aug.
Article
in En
| MEDLINE
| ID: mdl-2370112
Transmission electron microscopy was used to study ultrastructures in Paracoccidioides brasiliensis yeast cells after ingestion by nonactivated or cytokine-activated murine peritoneal macrophages. Yeast cells ingested by nonactivated macrophages had typical bi- and trilayered cell walls, plasma membranes, mitochondria, nuclei, vacuoles, etc., which remained intact for 24 h of coculture. In contrast, yeast cells ingested by activated macrophages exhibited abnormal mitochondrial ultrastructures within 4 h of interaction. Subsequent events that occurred were the formation of several clear vacuoles per cell, disintegration of the cytoplasm, and development of empty cells with intact walls. These findings provide, for the first time, insights into stepwise damage to fungal cells by activated macrophages (of particular interest in this instance because of prior evidence that the damage is due to nonoxidative mechanisms) and give possible clues regarding fungicidal mechanisms.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Paracoccidioides
/
Mitosporic Fungi
/
Macrophage Activation
/
Macrophages
Limits:
Animals
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Infect Immun
Year:
1990
Document type:
Article
Country of publication:
United States