Development and validation of a quantitation assay for fluorescently tagged HIV-1 virus-like particles.

Gutiérrez-Granados, Sonia; Cervera, Laura; Gòdia, Francesc; Carrillo, Jorge; Segura, María Mercedes

Gutiérrez-Granados, Sonia; Cervera, Laura; Gòdia, Francesc; Carrillo, Jorge; Segura, María Mercedes.

Affiliation

Gutiérrez-Granados S; Departament d'Enginyeria Química, Universitat Autònoma de Barcelona, campus Bellaterra, Cerdanyola del Vallès 08193, Barcelona, Spain.

J Virol Methods ; 193(1): 85-95, 2013 Oct.

Article in En | MEDLINE | ID: mdl-23707924

Subject(s)

HIV-1/isolation & purification; Staining and Labeling/methods; Viral Load/methods; Virosomes/isolation & purification; Fluorometry; Green Fluorescent Proteins/analysis; Green Fluorescent Proteins/genetics; HIV-1/genetics; Humans; Recombinant Fusion Proteins/analysis; Recombinant Fusion Proteins/genetics; Reproducibility of Results; Sensitivity and Specificity; Virosomes/genetics

Key words

Fluorescently tagged virus; Fluorometry; Gag-GFP; HIV-1 VLPs; Quantitation assays; Virus-like particles (VLPs)

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Staining and Labeling / HIV-1 / Viral Load / Virosomes Type of study: Diagnostic_studies / Guideline Limits: Humans Language: En Journal: J Virol Methods Year: 2013 Document type: Article Affiliation country: Spain Country of publication: Netherlands

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