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Distribution of Hepatitis C virus (HCV) genotypes in seropositive patients in the state of Alagoas, Brazil.
Gonzaga, Rosa Maria S; Rodart, Itatiana F; Reis, Mitermayer Galvão; Ramalho Neto, Cícero Eduardo; Silva, Denise Wanderlei.
Affiliation
  • Gonzaga RM; Laboratório de Genética Molecular, Genômica e Proteômica, Universidade Federal de Alagoas , Maceió, AL , Brasil.
Braz J Microbiol ; 39(4): 644-7, 2008 Oct.
Article in En | MEDLINE | ID: mdl-24031281
We determined the frequency of hepatitis C virus (HCV) genotypes in anti-HCV seropositive patients in the state of Alagoas, Brazil, by means of nested-reverse transcription-polymerase chain reaction (RT-nested-PCR) followed by restriction fragment length polymorphism (RFLP) of amplified fragments of the 5´NCR. The nested-PCR with genotype-specific primers from the core region was carried out when detection was not possible by the first approach. Detectable HCV-RNA was present in 115 (74.7%) of 154 serum samples. Genotype 1 was the most frequent (77.4%), against 20.9% of genotype 3 and 0.8% of genotype 2. Subtype 1b was predominant (65.2%), followed by subtypes 1a (8.7%), and 3a (6.1%). Coinfection (1a/3a) was detected in 0.8% of the samples. Indeed, there was no significant differences in the prevalence of genotype 1 compared to what has been obtained from anti-HCV seropositive patients from other locations in Brazil. Here we report for the first time the genotype 2 in the state of Alagoas.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Risk_factors_studies Country/Region as subject: America do sul / Brasil Language: En Journal: Braz J Microbiol Year: 2008 Document type: Article Affiliation country: Brazil Country of publication: Brazil

Full text: 1 Collection: 01-internacional Database: MEDLINE Type of study: Risk_factors_studies Country/Region as subject: America do sul / Brasil Language: En Journal: Braz J Microbiol Year: 2008 Document type: Article Affiliation country: Brazil Country of publication: Brazil