A new and improved method based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) for the determination of A1298C mutation in the methylenetetrahydrofolate reductase (MTHFR) gene.

Machnik, Grzegorz; Zapala, Malgorzata; Pelc, Ewa; Gasecka-Czapla, Monika; Kaczmarczyk, Grzegorz; Okopien, Boguslaw

Machnik, Grzegorz; Zapala, Malgorzata; Pelc, Ewa; Gasecka-Czapla, Monika; Kaczmarczyk, Grzegorz; Okopien, Boguslaw.

Affiliation

Machnik G; Department of Pharmacology, Medical University of Silesia, Katowice, Medyków 18, 40-752 Katowice, Poland; phone: +48 32 208 85 17; fax: + 48 32 252 3902; e mail: gmachnik@sum.edu.pl.

Ann Clin Lab Sci ; 43(4): 436-40, 2013.

Article in En | MEDLINE | ID: mdl-24247802

Subject(s)

Methylenetetrahydrofolate Reductase (NADPH2)/genetics; Molecular Biology/methods; Mutation, Missense/genetics; Polymerase Chain Reaction/methods; Polymorphism, Restriction Fragment Length/genetics; Base Sequence; Genotype; Humans; Molecular Sequence Data

Key words

Methylenetetrahydrofolate reductase; Polymorphism; molecular biology; mutation; restriction fragment length

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Collection: 01-internacional Database: MEDLINE Main subject: Polymorphism, Restriction Fragment Length / Polymerase Chain Reaction / Mutation, Missense / Methylenetetrahydrofolate Reductase (NADPH2) / Molecular Biology Limits: Humans Language: En Journal: Ann Clin Lab Sci Year: 2013 Document type: Article Country of publication: United States

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