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Differential expression of the MHM region and of sex-determining-related genes during gonadal development in chicken embryos.
Caetano, L C; Gennaro, F G O; Coelho, K; Araújo, F M; Vila, R A; Araújo, A; de Melo Bernardo, A; Marcondes, C R; Chuva de Sousa Lopes, S M; Ramos, E S.
Affiliation
  • Caetano LC; Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
  • Gennaro FG; Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
  • Coelho K; Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
  • Araújo FM; Departamento de Ginecologia e Obstetrícia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
  • Vila RA; Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
  • Araújo A; Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
  • de Melo Bernardo A; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.
  • Marcondes CR; Embrapa Pecuária Sudeste, São Carlos, SP, Brasil.
  • Chuva de Sousa Lopes SM; Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, The Netherlands.
  • Ramos ES; Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil esramos@usp.br.
Genet Mol Res ; 13(1): 838-49, 2014 Feb 13.
Article in En | MEDLINE | ID: mdl-24615048
The chicken (Gallus gallus) embryo has been used as a classic model system for developmental studies because of its easy accessibility for surgical manipulation during embryonic development. Sex determination in birds is chromosomally based (ZZ for males and ZW for females); however, the basic mechanism of sex determination is still unknown. Here, the dynamics of expression of candidate genes implicated in vertebrate sex determination and differentiation were studied during embryonic chicken gonadal development. Gene expression profiles were obtained before, during, and after gonadal sex differentiation in females and males for DMRT1, SOX3, SOX9, DAX1, SCII, HINTZ, HINTW, and the male hypermethylated (MHM) region. Transcripts for the HINTZ, DMRT1, DAX1, SCII, and SOX9 genes were observed in both sexes, but expression was higher in male gonads and may be correlated with testicular differentiation. The expression patterns of HINTW, SOX3, and MHM suggest that they may act in ovary development and may be involved in meiosis entry. MHM was upregulated and DMRT1 was downregulated in females at the same developmental stage. This may indicate a regulation of DMRT1 by MHM ncRNA. Similar dynamics were observed between HINTW and HINTZ. This study reports on the MHM expression profile during gonadal development and its correlation with the expression of genes involved in vertebrate sex determination.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sex Differentiation / Sex Determination Processes / Gonads Limits: Animals Language: En Journal: Genet Mol Res Journal subject: BIOLOGIA MOLECULAR / GENETICA Year: 2014 Document type: Article Affiliation country: Brazil Country of publication: Brazil
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sex Differentiation / Sex Determination Processes / Gonads Limits: Animals Language: En Journal: Genet Mol Res Journal subject: BIOLOGIA MOLECULAR / GENETICA Year: 2014 Document type: Article Affiliation country: Brazil Country of publication: Brazil