Short communication: development and characterization of novel transcriptome-derived microsatellites for genetic analysis of persimmon.
Genet Mol Res
; 13(2): 3013-24, 2014 Apr 16.
Article
in En
| MEDLINE
| ID: mdl-24782136
Oriental persimmon (Diospyros kaki Thunb.) (2n = 6x = 90) is a major commercial and deciduous fruit tree that is believed to have originated in China. However, rare transcriptomic and genomic information on persimmon is available. Using Roche 454 sequencing technology, the transcriptome from RNA of the flowers of D. kaki was analyzed. A total of 1,250,893 reads were generated and 83,898 unigenes were assembled. A total of 42,711 SSR loci were identified from 23,494 unigenes and 289 polymerase chain reaction primer pairs were designed. Of these 289 primers, 155 (53.6%) showed robust PCR amplification and 98 revealed polymorphism between 15 persimmon genotypes, indicating a polymorphic rate of 63.23% of the productive primers for characterization and genotyping of the genus Diospyros. Transcriptome sequence data generated from next-generation sequencing technology to identify microsatellite loci appears to be rapid and cost-efficient, particularly for species with no genomic sequence information available.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Microsatellite Repeats
/
Diospyros
/
Transcriptome
Limits:
Humans
Country/Region as subject:
Asia
Language:
En
Journal:
Genet Mol Res
Journal subject:
BIOLOGIA MOLECULAR
/
GENETICA
Year:
2014
Document type:
Article
Affiliation country:
China
Country of publication:
Brazil