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Stepwise differentiation of pluripotent stem cells into osteoblasts using four small molecules under serum-free and feeder-free conditions.
Kanke, Kosuke; Masaki, Hideki; Saito, Taku; Komiyama, Yuske; Hojo, Hironori; Nakauchi, Hiromitsu; Lichtler, Alexander C; Takato, Tsuyoshi; Chung, Ung-Il; Ohba, Shinsuke.
Affiliation
  • Kanke K; Center for Disease Biology and Integrative Medicine, The University of Tokyo, Tokyo 113-0033, Japan ; Department of Sensory and Motor System Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
  • Masaki H; Japan Science Technology Agency, ERATO, Nakauchi Stem Cell and Organ Regeneration Project, The University of Tokyo, Tokyo 108-8639, Japan ; Division of Stem Cell Therapy, Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Ja
  • Saito T; Department of Sensory and Motor System Medicine, The University of Tokyo, Tokyo 113-0033, Japan ; Department of Bone and Cartilage Regenerative Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
  • Komiyama Y; Intensive Care Unit, The University of Tokyo, Tokyo 113-0033, Japan.
  • Hojo H; Center for Disease Biology and Integrative Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
  • Nakauchi H; Japan Science Technology Agency, ERATO, Nakauchi Stem Cell and Organ Regeneration Project, The University of Tokyo, Tokyo 108-8639, Japan ; Division of Stem Cell Therapy, Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Ja
  • Lichtler AC; Department of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, Farmington, CT 06030, USA.
  • Takato T; Department of Sensory and Motor System Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
  • Chung UI; Center for Disease Biology and Integrative Medicine, The University of Tokyo, Tokyo 113-0033, Japan ; Department of Bioengineering, The University of Tokyo, Tokyo 113-0033, Japan.
  • Ohba S; Center for Disease Biology and Integrative Medicine, The University of Tokyo, Tokyo 113-0033, Japan ; Department of Bioengineering, The University of Tokyo, Tokyo 113-0033, Japan.
Stem Cell Reports ; 2(6): 751-60, 2014 Jun 03.
Article in En | MEDLINE | ID: mdl-24936463
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Osteoblasts / Culture Media, Serum-Free / Cell Culture Techniques / Pluripotent Stem Cells Limits: Animals / Humans Language: En Journal: Stem Cell Reports Year: 2014 Document type: Article Affiliation country: Japan Country of publication: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Osteoblasts / Culture Media, Serum-Free / Cell Culture Techniques / Pluripotent Stem Cells Limits: Animals / Humans Language: En Journal: Stem Cell Reports Year: 2014 Document type: Article Affiliation country: Japan Country of publication: United States