Development of a versatile and stable internal control system for RT-qPCR assays.

Felder, Eva; Wölfel, Roman

Felder, Eva; Wölfel, Roman.

Affiliation

Felder E; Bundeswehr Institute of Microbiology, Department for Medical Bio Reconnaissance and Verification, Neuherbergstr. 11, 80937 Munich, Germany. Electronic address: evafelder@bundeswehr.org.
Wölfel R; Bundeswehr Institute of Microbiology, Department for Medical Bio Reconnaissance and Verification, Neuherbergstr. 11, 80937 Munich, Germany.

J Virol Methods ; 208: 33-40, 2014 Nov.

Article in En | MEDLINE | ID: mdl-25072380

Subject(s)

Real-Time Polymerase Chain Reaction/standards; Reference Standards; Reverse Transcriptase Polymerase Chain Reaction/standards; Virosomes/genetics; Escherichia coli/genetics; Freezing; Orthohantavirus/genetics; Hemorrhagic Fever Virus, Crimean-Congo/genetics; Levivirus/genetics; RNA Stability; Virosomes/isolation & purification; Virosomes/radiation effects

Key words

Crimean-Congo Hemorrhagic Fever Virus; Field application; Hantavirus; MS2-based VLPs; RT-qPCR; Stable internal control

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Reference Standards / Reverse Transcriptase Polymerase Chain Reaction / Virosomes / Real-Time Polymerase Chain Reaction Type of study: Evaluation_studies Language: En Journal: J Virol Methods Year: 2014 Document type: Article Country of publication: Netherlands

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