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Effect of γ-irradiation on expression of tight and adherens junction protein mRNA on in vitro blood-brain barrier model.
Zorkina, Ya A; Volgina, N E; Gorlachev, G E; Mel'nikov, P A; Golanov, A V; Potapov, A A; Chekhonin, V P.
Affiliation
  • Zorkina YA; V. P. Serbsky State Research Center for Social and Forensic Psychiatry, Moscow, Russia, zrkyana@gmail.com.
Bull Exp Biol Med ; 158(1): 127-36, 2014 Nov.
Article in En | MEDLINE | ID: mdl-25408523
We studied the effect of γ-irradiation on HUVEC endothelial cells co-cultured with allogeneic astrocytes. This 2D in vitro model of the blood-brain barrier has the same parameters as cerebral microvascular endothelial cells forming the blood-brain barrier and allows reproducing its functions in vivo. Dose-dependent changes in cell morphology and violation of monolayer integrity were observed. Real-time PCR and immunocytochemical analysis revealed changes in the expression of tight (ZO-1, claudin-5) and adherens junction protein (vascular endothelial cadherin, ß-catenin) mRNA. Expression of tight and adherens junction proteins mRNA decreased in 2, 24, and 48 h after irradiation in doses of 2, 4, and 6 Gy. Significant dose-dependent changes were found only for ß-catenin mRNA expression in 2 h after exposition. This model of blood-brain barrier in vitro can be used for studying the molecular mechanisms regulating permeability of cerebral endothelium under normal conditions and after pathological exposures, e.g. γ-irradiation.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Blood-Brain Barrier / Gene Expression / Tight Junctions / Adherens Junctions / Human Umbilical Vein Endothelial Cells / Gamma Rays Limits: Humans Language: En Journal: Bull Exp Biol Med Year: 2014 Document type: Article Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Blood-Brain Barrier / Gene Expression / Tight Junctions / Adherens Junctions / Human Umbilical Vein Endothelial Cells / Gamma Rays Limits: Humans Language: En Journal: Bull Exp Biol Med Year: 2014 Document type: Article Country of publication: United States