Effect of γ-irradiation on expression of tight and adherens junction protein mRNA on in vitro blood-brain barrier model.
Bull Exp Biol Med
; 158(1): 127-36, 2014 Nov.
Article
in En
| MEDLINE
| ID: mdl-25408523
We studied the effect of γ-irradiation on HUVEC endothelial cells co-cultured with allogeneic astrocytes. This 2D in vitro model of the blood-brain barrier has the same parameters as cerebral microvascular endothelial cells forming the blood-brain barrier and allows reproducing its functions in vivo. Dose-dependent changes in cell morphology and violation of monolayer integrity were observed. Real-time PCR and immunocytochemical analysis revealed changes in the expression of tight (ZO-1, claudin-5) and adherens junction protein (vascular endothelial cadherin, ß-catenin) mRNA. Expression of tight and adherens junction proteins mRNA decreased in 2, 24, and 48 h after irradiation in doses of 2, 4, and 6 Gy. Significant dose-dependent changes were found only for ß-catenin mRNA expression in 2 h after exposition. This model of blood-brain barrier in vitro can be used for studying the molecular mechanisms regulating permeability of cerebral endothelium under normal conditions and after pathological exposures, e.g. γ-irradiation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blood-Brain Barrier
/
Gene Expression
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Tight Junctions
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Adherens Junctions
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Human Umbilical Vein Endothelial Cells
/
Gamma Rays
Limits:
Humans
Language:
En
Journal:
Bull Exp Biol Med
Year:
2014
Document type:
Article
Country of publication:
United States