The in vitro PIG-A gene mutation assay: mutagenicity testing via flow cytometry based on the glycosylphosphatidylinositol (GPI) status of TK6 cells.

Krüger, Christopher T; Hofmann, Mareike; Hartwig, Andrea

Krüger, Christopher T; Hofmann, Mareike; Hartwig, Andrea.

Affiliation

Krüger CT; Department of Food Chemistry and Toxicology, Institute for Applied Biosciences, Karlsruhe Institute of Technology (KIT), Kaiserstrasse 12, 76131, Karlsruhe, Germany.
Hofmann M; Department of Food Chemistry and Toxicology, Institute for Applied Biosciences, Karlsruhe Institute of Technology (KIT), Kaiserstrasse 12, 76131, Karlsruhe, Germany.
Hartwig A; Department of Food Chemistry and Toxicology, Institute for Applied Biosciences, Karlsruhe Institute of Technology (KIT), Kaiserstrasse 12, 76131, Karlsruhe, Germany. andrea.hartwig@kit.edu.

Arch Toxicol ; 89(12): 2429-43, 2015 Dec.

Article in En | MEDLINE | ID: mdl-25417052

Subject(s)

Flow Cytometry/methods; Glycosylphosphatidylinositols/genetics; Membrane Proteins/genetics; Mutagens/toxicity; 4-Nitroquinoline-1-oxide/administration & dosage; 4-Nitroquinoline-1-oxide/toxicity; B-Lymphocytes/drug effects; Cell Line; Dose-Response Relationship, Drug; Ethyl Methanesulfonate/administration & dosage; Ethyl Methanesulfonate/toxicity; Glycosylphosphatidylinositols/deficiency; Humans; Male; Mutagenicity Tests/methods; Mutagens/administration & dosage; Mutation; Ultraviolet Rays/adverse effects

Key words

Flow cytometry; GPI; Gene mutation assay; PIG-A; TK6

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Glycosylphosphatidylinositols / Flow Cytometry / Membrane Proteins / Mutagens Limits: Humans / Male Language: En Journal: Arch Toxicol Year: 2015 Document type: Article Affiliation country: Germany Country of publication: Germany

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