Parafilm-assisted microdissection: a sampling method for mass spectrometry-based identification of differentially expressed prostate cancer protein biomarkers.
Chem Commun (Camb)
; 51(22): 4564-7, 2015 Mar 18.
Article
in En
| MEDLINE
| ID: mdl-25490716
Mass spectrometry-based methods for prostate cancer biomarker discovery are hampered by their low-throughput capabilities because of extensive sample preparation. We present the parafilm-assisted microdissection technique coupled with label-free quantification and bioinformatics analysis as a means to evaluate directly protein expression changes on benign and tumor regions.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Paraffin
/
Prostatic Neoplasms
/
Mass Spectrometry
/
Biomarkers, Tumor
/
Microdissection
/
Neoplasm Proteins
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
/
Male
Language:
En
Journal:
Chem Commun (Camb)
Journal subject:
QUIMICA
Year:
2015
Document type:
Article
Affiliation country:
France
Country of publication:
United kingdom