[Rapid serologic diagnosis of Argentinian hemorrhagic fever in whole blood]. / Diagnóstico serológico rápido de fiebre hemorrágica Argentina en sangre entera.
Rev Argent Microbiol
; 21(2): 85-8, 1989.
Article
in Es
| MEDLINE
| ID: mdl-2559426
The usefulness of a method for detection of antibodies against Junin virus in whole blood was tested. N: NIH adult mice were inoculated with 10(3) PFU of attenuated XJ-Clon 3 Junin virus strain by intraperitoneal route and blood was obtained by retro-orbital puncture at 21 days post-infection. One blood aliquot (50 microliters) was collected in tubes containing a stabilizer solution for whole blood and another was processed for serum obtention. Immunofluorescent antibodies were tested on spot slides of a BHK/21 cell line persistently infected with Junin virus. High antibody titers (1/64 to 1/256) were detected in both whole blood and serum, with 66% coincidence between both procedures. These results show that the method of detection of antibodies in whole blood would be useful to test quickly for anti-Junin virus antibodies in seroepidemiologic studies, in endemic areas.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Arenaviridae
/
Arenaviruses, New World
/
Hemorrhagic Fever, American
/
Antibodies, Viral
Type of study:
Diagnostic_studies
Limits:
Animals
Country/Region as subject:
America do sul
/
Argentina
Language:
Es
Journal:
Rev Argent Microbiol
Year:
1989
Document type:
Article
Affiliation country:
Argentina
Country of publication:
Argentina