Molecular mutagenesis at Tyr-101 of the amylomaltase transcribed from a gene isolated from soil DNA.

Watanasatitarpa, S; Rudeekulthamrong, P; Krusong, K; Srisimarat, W; Zimmermann, W; Pongsawasdi, P; Kaulpiboon, J

Watanasatitarpa, S; Rudeekulthamrong, P; Krusong, K; Srisimarat, W; Zimmermann, W; Pongsawasdi, P; Kaulpiboon, J.

Prikl Biokhim Mikrobiol ; 50(3): 273-82, 2014.

Article in En | MEDLINE | ID: mdl-25757335

Subject(s)

Bacterial Proteins/genetics; Cyclodextrins/biosynthesis; DNA, Bacterial/genetics; Glycogen Debranching Enzyme System/genetics; Mutagenesis, Site-Directed; Transcription, Genetic; Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/isolation & purification; Bacterial Proteins/metabolism; Cloning, Molecular; Cyclization; DNA, Bacterial/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Glycogen Debranching Enzyme System/chemistry; Glycogen Debranching Enzyme System/isolation & purification; Glycogen Debranching Enzyme System/metabolism; Hydrogen-Ion Concentration; Molecular Sequence Data; Oligosaccharides/metabolism; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Sequence Homology, Amino Acid; Soil/chemistry; Substrate Specificity; Thermus thermophilus/enzymology; Thermus thermophilus/genetics; Trisaccharides/metabolism

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Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Transcription, Genetic / DNA, Bacterial / Glycogen Debranching Enzyme System / Mutagenesis, Site-Directed / Cyclodextrins Language: En Journal: Prikl Biokhim Mikrobiol Year: 2014 Document type: Article Country of publication: RUSSIA

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