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CRISPR/Cas9 nuclease cleavage combined with Gibson assembly for seamless cloning.
Wang, Jia-Wang; Wang, Amy; Li, Kunyu; Wang, Bangmei; Jin, Shunqian; Reiser, Michelle; Lockey, Richard F.
Affiliation
  • Wang JW; Division of Allergy and Immunology, Department of Internal Medicine, University of South Florida Morsani College of Medicine, Tampa, FL.
  • Wang A; Division of Allergy and Immunology, Department of Internal Medicine, University of South Florida Morsani College of Medicine, Tampa, FL.
  • Li K; Division of Allergy and Immunology, Department of Internal Medicine, University of South Florida Morsani College of Medicine, Tampa, FL.
  • Wang B; Division of Allergy and Immunology, Department of Internal Medicine, University of South Florida Morsani College of Medicine, Tampa, FL.
  • Jin S; Department of Pediatrics, Children's Hospital of Pittsburgh of University of Pittsburgh Medical Center and the University of Pittsburgh School of Medicine, Pittsburgh, PA.
  • Reiser M; Division of Allergy and Immunology, Department of Internal Medicine, University of South Florida Morsani College of Medicine, Tampa, FL.
  • Lockey RF; Division of Allergy and Immunology, Department of Internal Medicine, University of South Florida Morsani College of Medicine, Tampa, FL.
Biotechniques ; 58(4): 161-70, 2015 Apr.
Article in En | MEDLINE | ID: mdl-25861928
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Cloning, Molecular / Endonucleases / DNA Cleavage / CRISPR-Associated Proteins / CRISPR-Cas Systems Type of study: Evaluation_studies Limits: Animals Language: En Journal: Biotechniques Year: 2015 Document type: Article Country of publication: United kingdom
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Cloning, Molecular / Endonucleases / DNA Cleavage / CRISPR-Associated Proteins / CRISPR-Cas Systems Type of study: Evaluation_studies Limits: Animals Language: En Journal: Biotechniques Year: 2015 Document type: Article Country of publication: United kingdom