Photolytic Cross-Linking to Probe Protein-Protein and Protein-Matrix Interactions in Lyophilized Powders.

Iyer, Lavanya K; Moorthy, Balakrishnan S; Topp, Elizabeth M

Iyer, Lavanya K; Moorthy, Balakrishnan S; Topp, Elizabeth M.

Affiliation

Iyer LK; Department of Industrial and Physical Pharmacy, Purdue University , West Lafayette, Indiana 47907-2091, United States.
Moorthy BS; Department of Industrial and Physical Pharmacy, Purdue University , West Lafayette, Indiana 47907-2091, United States.
Topp EM; Department of Industrial and Physical Pharmacy, Purdue University , West Lafayette, Indiana 47907-2091, United States.

Mol Pharm ; 12(9): 3237-49, 2015 Sep 08.

Article in En | MEDLINE | ID: mdl-26204425

Subject(s)

Cross-Linking Reagents/metabolism; Excipients/chemistry; Freeze Drying/methods; Myoglobin/metabolism; Peptide Fragments/metabolism; Photolysis; Powders/chemistry; Animals; Chemistry, Pharmaceutical; Chromatography, Liquid/methods; Guanidine/chemistry; Horses; Mass Spectrometry/methods; Myoglobin/chemistry; Peptide Fragments/chemistry; Water/chemistry

Key words

lyophilized formulations; mass spectrometry; myoglobin; photolytic cross-linking; protein−excipient interactions; protein−protein interactions; protein−water interactions; raffinose; succinimidyl 4,4'-azipentanoate

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptide Fragments / Photolysis / Powders / Cross-Linking Reagents / Excipients / Freeze Drying / Myoglobin Limits: Animals Language: En Journal: Mol Pharm Journal subject: BIOLOGIA MOLECULAR / FARMACIA / FARMACOLOGIA Year: 2015 Document type: Article Affiliation country: United States Country of publication: United States

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