Ki67/KIT double immunohistochemical staining in cutaneous mast cell tumors from Boxer dogs.
Res Vet Sci
; 102: 122-6, 2015 Oct.
Article
in En
| MEDLINE
| ID: mdl-26412531
Cutaneous mast cell tumors (MCTs) are among the most frequent malignant tumors in dogs and Boxer breed dogs have a higher incidence of this disease. Ki67 staining and KIT staining are widely used to predict natural behavior in canine MCT but no previous study has evaluated double staining of these proteins as a prognostic factor. Based on biological behavior predictors in canine MCT, the purpose of this study was to determine the Ki67 proliferative index in KIT positive cells using double stain immunohistochemistry technique. Sixty-nine MCTs from Boxer dogs were selected and a tissue microarray was constructed for the double stained immunohistochemistry. Double positivity (Ki67(+)/KIT(+)) was observed in 20/69 (29%) MCT, with a mean of 9.06 double positive cells per tissue core (range 0.48%-43.97%) and Ki67(-)/KIT(+) animals had a longer survival time than Ki67(+)/KIT(+) animals (p=0.03).
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Proto-Oncogene Proteins c-kit
/
Ki-67 Antigen
/
Mastocytoma
/
Dog Diseases
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Res Vet Sci
Year:
2015
Document type:
Article
Affiliation country:
Brazil
Country of publication:
United kingdom