Rapid screening of phytosterols in orange juice by solid-phase microextraction on polyacrylate fibre derivatisation and gas chromatographic-mass spectrometric.
Food Chem
; 132(1): 613-8, 2012 May 01.
Article
in En
| MEDLINE
| ID: mdl-26434339
ABSTRACT
The potential of solid-phase microextraction on polyacrylate coated fibre, with sequential or simultaneous trimethylsilyl derivatisation followed by gas chromatographic-mass spectrometric analysis, was evaluated for a rapid determination of the distribution of the phytosterols in aqueous food matrixes. Influences of different parameters (bis(trimethylsilyl)trifluoro-acetamide and sterol exposure time, sterol concentration and experimental protocol) on the recovery of sterols were investigated to determine optimum conditions which were tested for sterol extraction and analysis from orange juice. Best selectivity, sterol recovery and derivatisation yields were obtained by extraction and simultaneous derivatisation through immersion of the SPME-PA fibre in the orange juice (10min, 65°C) after headspace absorption of BSTFA (30min, 65°C) on the fibre. Nevertheless the method developed cannot be used for quantitative analysis. But the possibility to effect rapid screen of phytosterol containing in complex media have been shown.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phytosterols
/
Citrus sinensis
/
Solid Phase Microextraction
/
Gas Chromatography-Mass Spectrometry
Type of study:
Diagnostic_studies
/
Screening_studies
Language:
En
Journal:
Food Chem
Year:
2012
Document type:
Article