Your browser doesn't support javascript.
loading
Effects and mechanism of lipoic acid on beta-amyloid-intoxicated C6 glioma cells.
Xing, Z G; Yu, G D; Qin, L; Jiang, F; Zhao, W H.
Affiliation
  • Xing ZG; Department of Neurosurgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China.
  • Yu GD; Neurosurgery Department of Xianning Central Hospital, Xianning, Hubei Province, China.
  • Qin L; Neurosurgery Department of Xianning Central Hospital, Xianning, Hubei Province, China.
  • Jiang F; Neurosurgery Department, First Affiliated Hospital of Hunan Traditional Chinese Medical College, Zhuzhou, Hunan Province, China.
  • Zhao WH; Neurosurgery Department of Xianning Central Hospital, Xianning, Hubei Province, China.
Genet Mol Res ; 14(4): 13880-8, 2015 Oct 30.
Article in En | MEDLINE | ID: mdl-26535703
ß-amyloid peptides (Aßs) can exert neurotoxic effects through induction of oxidative damage, whereas lipoic acid (LA), a powerful antioxidant, can alleviate oxidative damage. In this study, we explored the effect and mechanism of action of LA on beta-amyloid-intoxicated C6 glioma cells. Cells were randomly divided into three groups: control (vehicle), Aß, and LA + Aß. The LA + Aß group was treated with LA for 2 h, then both the Aß-only and the LA + Aß groups were incubated with 25 µM Aß for 24 h. Cell viability was measured by the MTT method. Mitochondrial reduced glutathione (GSH) and oxidized glutathione (GSSG) levels were detected by enzyme-linked immunosorbent assay (ELISA), and the GSH to GSSG ratio calculated. Real-time polymerase chain reaction and western blot analyses were used to detect MnSOD mRNA and protein, respectively. Aß significantly inhibited C6 cell proliferation compared with the control group (P < 0.05). LA markedly increased cell viability compared with the Aß group (P < 0.05). The increased GSSH and decreased GSH mitochondrial accumulation induced by Aß was profoundly reversed by treatment with LA (P < 0.05). Aß significantly reduced MnSOD expression compared to controls (P < 0.05), whereas LA pretreatment increased MnSOD expression compared with the Aß-only group (P < 0.05); MnSOD protein levels showed similar patterns. These results suggest that LA might protect Aß-intoxicated C6 glioma cells by alleviating oxidative damage, providing a new treatment strategy for neurodegenerative diseases.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Amyloid beta-Peptides / Thioctic Acid / Glioma / Antioxidants Limits: Animals Language: En Journal: Genet Mol Res Journal subject: BIOLOGIA MOLECULAR / GENETICA Year: 2015 Document type: Article Affiliation country: China Country of publication: Brazil

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Amyloid beta-Peptides / Thioctic Acid / Glioma / Antioxidants Limits: Animals Language: En Journal: Genet Mol Res Journal subject: BIOLOGIA MOLECULAR / GENETICA Year: 2015 Document type: Article Affiliation country: China Country of publication: Brazil