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Optimized Protocol for Isolation of Multipotent Mesenchymal Stromal Cells from Human Umbilical Cord.
Romanov, Yu A; Balashova, E E; Volgina, N E; Kabaeva, N V; Dugina, T N; Sukhikh, G T.
Affiliation
  • Romanov YA; Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia. romanov@cardio.ru.
  • Balashova EE; CryoCenter Umbilical Cord Blood Bank, Moscow, Russia. romanov@cardio.ru.
  • Volgina NE; Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia.
  • Kabaeva NV; CryoCenter Umbilical Cord Blood Bank, Moscow, Russia.
  • Dugina TN; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia.
  • Sukhikh GT; Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med ; 160(1): 148-54, 2015 Nov.
Article in En | MEDLINE | ID: mdl-26597687
Extraembryonic tissues, in particular, umbilical cord stroma are promising sources of multipotent mesenchymal stromal cells for regenerative medicine. In recent years, methods for isolation of mesenchymal stromal cells from different compartments of the umbilical cords based on enzymatic disaggregation of the tissue or on tissue explants have been proposed. Here we propose a protocol of isolation of multipotent mesenchymal stromal cells from the whole umbilical cord that combines the advantages of each approach and ensures sufficient cell yield for further experimental and clinical applications. A combination of short-term incubation of tissue fragments on cold collagenase solution followed by their culturing in the form of explants significantly increased the yield of cells with high proliferative activity, typical pluripotent mesenchymal stromal cell phenotype, and preserved differentiation capacity.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Umbilical Cord / Cell Separation / Stromal Cells / Pluripotent Stem Cells / Mesenchymal Stem Cells Type of study: Guideline Limits: Humans / Newborn Language: En Journal: Bull Exp Biol Med Year: 2015 Document type: Article Affiliation country: Russia Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Umbilical Cord / Cell Separation / Stromal Cells / Pluripotent Stem Cells / Mesenchymal Stem Cells Type of study: Guideline Limits: Humans / Newborn Language: En Journal: Bull Exp Biol Med Year: 2015 Document type: Article Affiliation country: Russia Country of publication: United States