Optimized Protocol for Isolation of Multipotent Mesenchymal Stromal Cells from Human Umbilical Cord.
Bull Exp Biol Med
; 160(1): 148-54, 2015 Nov.
Article
in En
| MEDLINE
| ID: mdl-26597687
Extraembryonic tissues, in particular, umbilical cord stroma are promising sources of multipotent mesenchymal stromal cells for regenerative medicine. In recent years, methods for isolation of mesenchymal stromal cells from different compartments of the umbilical cords based on enzymatic disaggregation of the tissue or on tissue explants have been proposed. Here we propose a protocol of isolation of multipotent mesenchymal stromal cells from the whole umbilical cord that combines the advantages of each approach and ensures sufficient cell yield for further experimental and clinical applications. A combination of short-term incubation of tissue fragments on cold collagenase solution followed by their culturing in the form of explants significantly increased the yield of cells with high proliferative activity, typical pluripotent mesenchymal stromal cell phenotype, and preserved differentiation capacity.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Umbilical Cord
/
Cell Separation
/
Stromal Cells
/
Pluripotent Stem Cells
/
Mesenchymal Stem Cells
Type of study:
Guideline
Limits:
Humans
/
Newborn
Language:
En
Journal:
Bull Exp Biol Med
Year:
2015
Document type:
Article
Affiliation country:
Russia
Country of publication:
United States