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Fatty acid analysis of triacylglycerols: Preparation of fatty acid methyl esters for gas chromatography.
Ichihara, Ken'ichi; Kohsaka, Chihiro; Tomari, Naohiro; Kiyono, Tamami; Wada, Jun; Hirooka, Kiyoo; Yamamoto, Yoshihiro.
Affiliation
  • Ichihara K; Kyoto Integrated Science & Technology Bio-Analysis Center, Advanced Science, Technology & Management Research Institute of Kyoto (ASTEM), Kyoto 600-8813, Japan. Electronic address: ichihara@kpu.ac.jp.
  • Kohsaka C; Kyoto Municipal Institute of Industrial Technology and Culture, Kyoto 600-8815, Japan.
  • Tomari N; Kyoto Municipal Institute of Industrial Technology and Culture, Kyoto 600-8815, Japan.
  • Kiyono T; Kyoto Municipal Institute of Industrial Technology and Culture, Kyoto 600-8815, Japan.
  • Wada J; Kyoto Municipal Institute of Industrial Technology and Culture, Kyoto 600-8815, Japan.
  • Hirooka K; Kyoto Municipal Institute of Industrial Technology and Culture, Kyoto 600-8815, Japan.
  • Yamamoto Y; Kyoto Municipal Institute of Industrial Technology and Culture, Kyoto 600-8815, Japan.
Anal Biochem ; 495: 6-8, 2016 Feb 15.
Article in En | MEDLINE | ID: mdl-26656926
A method to prepare fatty acid methyl esters was developed for fatty acid analysis of triacylglycerols by gas chromatography (GC). Triacylglycerols were mixed with methanolic CH3ONa in hexane containing a mid-polar solvent for 10 s at room temperature. Under these conditions, trioleoylglycerol was converted to methyl oleate with an average yield of 99.3%. This procedure gave reliable and reproducible data on fatty acid compositions determined by GC.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Triglycerides / Chromatography, Gas / Fatty Acids Language: En Journal: Anal Biochem Year: 2016 Document type: Article Country of publication: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Triglycerides / Chromatography, Gas / Fatty Acids Language: En Journal: Anal Biochem Year: 2016 Document type: Article Country of publication: United States