Characterization of a SILAC method for proteomic analysis of primary rat microglia.

Zhang, Ping; Culver-Cochran, Ashley E; Stevens, Stanley M; Liu, Bin

Zhang, Ping; Culver-Cochran, Ashley E; Stevens, Stanley M; Liu, Bin.

Affiliation

Zhang P; Department of Pharmacodynamics, College of Pharmacy, University of Florida, Gainesville, FL, USA.
Culver-Cochran AE; Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida, Tampa, FL, USA.
Stevens SM; Department of Cell Biology, Microbiology, and Molecular Biology, University of South Florida, Tampa, FL, USA.
Liu B; Department of Pharmacodynamics, College of Pharmacy, University of Florida, Gainesville, FL, USA.

Proteomics ; 16(9): 1341-6, 2016 05.

Article in En | MEDLINE | ID: mdl-26936193

Subject(s)

Endotoxins/pharmacology; Isotope Labeling/methods; Microglia/drug effects; Models, Neurological; Nerve Tissue Proteins/genetics; Proteome/genetics; Animals; Animals, Newborn; Brain/cytology; Brain/drug effects; Brain/metabolism; Gene Expression; Gene Expression Profiling; Inflammation; Microglia/cytology; Microglia/metabolism; Primary Cell Culture; Proteome/metabolism; Rats; Rats, Sprague-Dawley

Key words

Cell biology; Mass spectrometry; Microglia; Neuroimmune; Pathway profiling; SILAC

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Microglia / Proteome / Endotoxins / Isotope Labeling / Models, Neurological / Nerve Tissue Proteins Limits: Animals Language: En Journal: Proteomics Journal subject: BIOQUIMICA Year: 2016 Document type: Article Affiliation country: United States Country of publication: Germany

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