Direct fluorescence detection of microRNA based on enzymatically engineered primer extension poly-thymine (EPEPT) reaction using copper nanoparticles as nano-dye.

Chi, Bao-Zhu; Liang, Ru-Ping; Qiu, Wei-Bin; Yuan, Yan-Hong; Qiu, Jian-Ding

Chi, Bao-Zhu; Liang, Ru-Ping; Qiu, Wei-Bin; Yuan, Yan-Hong; Qiu, Jian-Ding.

Affiliation

Chi BZ; College of Chemistry and Institute for Advanced Study, Nanchang University, Nanchang, Jiangxi 330031, China.
Liang RP; College of Chemistry and Institute for Advanced Study, Nanchang University, Nanchang, Jiangxi 330031, China. Electronic address: rpliang@ncu.edu.cn.
Qiu WB; College of Chemistry and Institute for Advanced Study, Nanchang University, Nanchang, Jiangxi 330031, China.
Yuan YH; College of Chemistry and Institute for Advanced Study, Nanchang University, Nanchang, Jiangxi 330031, China.
Qiu JD; College of Chemistry and Institute for Advanced Study, Nanchang University, Nanchang, Jiangxi 330031, China. Electronic address: jdqiu@ncu.edu.cn.

Biosens Bioelectron ; 87: 216-221, 2017 Jan 15.

Article in En | MEDLINE | ID: mdl-27566394

Subject(s)

Biosensing Techniques/methods; Copper/chemistry; Fluorescent Dyes/chemistry; Metal Nanoparticles/chemistry; MicroRNAs/analysis; Poly T/chemistry; A549 Cells; DNA Nucleotidylexotransferase/chemistry; DNA Polymerase I/chemistry; Fluorescence; Humans; Limit of Detection; MCF-7 Cells; Spectrometry, Fluorescence/methods

Key words

Fluorescence detection; KFexo(−); PolyT-CuNPs; TdTase; miRNA

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Poly T / Biosensing Techniques / Copper / MicroRNAs / Metal Nanoparticles / Fluorescent Dyes Type of study: Diagnostic_studies Limits: Humans Language: En Journal: Biosens Bioelectron Journal subject: BIOTECNOLOGIA Year: 2017 Document type: Article Affiliation country: China

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