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Screening of filamentous fungi to produce xylanase and xylooligosaccharides in submerged and solid-state cultivations on rice husk, soybean hull, and spent malt as substrates.
da Silva Menezes, Bruna; Rossi, Daniele Misturini; Ayub, Marco Antônio Záchia.
Affiliation
  • da Silva Menezes B; Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil.
  • Rossi DM; Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil.
  • Ayub MA; Biotechnology, Bioprocess, and Biocatalysis Group, Food Science and Technology Institute, Federal University of Rio Grande do Sul, Av. Bento Gonçalves 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil. mazayub@ufrgs.br.
World J Microbiol Biotechnol ; 33(3): 58, 2017 Mar.
Article in En | MEDLINE | ID: mdl-28238176
We investigated the enzymatic complex produced by selected fungi strains isolated from the environment using the agro-industrial residues rice husk, soybean hull, and spent malt as substrates. Microbial growth was carried out in solid-state cultivation (SSC) and in submerged cultivations (SC) and the enzymatic activities of xylanase, cellulase, ß-xylosidase, and ß-glucosidase were determined. All substrates were effective in inducing enzymatic activities, with one strain of Aspergillus brasiliensis BLf1 showing maximum activities for all enzymes, except for cellulases. Using this fungus, the enzymatic activities of xylanase, cellulase, and ß-glucosidase were generally higher in SSC compared to SC, producing maxima activities of 120.5, 25.3 and 47.4 U g-1 of dry substrate, respectively. ß-xylosidase activity of 28.1 U g-1 of dry substrate was highest in SC. Experimental design was carried out to optimize xylanase activity by A. brasiliensis BLf1 in SSC using rice husk as substrate, producing maximum xylanase activity 183.5 U g-1 dry substrate, and xylooligosaccharides were produced and characterized. These results suggest A. brasiliensis BLf1 can be used to produce important lytic enzymes to be applied in the preparation of xylooligosaccharides.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oligosaccharides / Oryza / Glycine max / Fungal Proteins / Fungi / Glucuronates Type of study: Diagnostic_studies / Screening_studies Language: En Journal: World J Microbiol Biotechnol Year: 2017 Document type: Article Affiliation country: Brazil Country of publication: Germany

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Oligosaccharides / Oryza / Glycine max / Fungal Proteins / Fungi / Glucuronates Type of study: Diagnostic_studies / Screening_studies Language: En Journal: World J Microbiol Biotechnol Year: 2017 Document type: Article Affiliation country: Brazil Country of publication: Germany