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Synthetic matrix of polyether-polyurethane as a biological platform for pancreatic regeneration.
Pereira, Luciana Xavier; Viana, Celso Tarso Rodrigues; Orellano, Laura Alejandra Ariza; Almeida, Simone Aparecida; Vasconcelos, Anilton Cesar; Goes, Alfredo de Miranda; Birbrair, Alexander; Andrade, Silvia Passos; Campos, Paula Peixoto.
Affiliation
  • Pereira LX; Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Viana CTR; Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Orellano LAA; Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Almeida SA; Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Vasconcelos AC; Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Goes AM; Department of Biochemistry and Immunology of Federal University Minas Gerais, Brazil.
  • Birbrair A; Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Andrade SP; Department of Physiology and Biophysics, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
  • Campos PP; Department of General Pathology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address: paulapc@icb.ufmg.br.
Life Sci ; 176: 67-74, 2017 May 01.
Article in En | MEDLINE | ID: mdl-28336399
AIMS: Several alternative cellular approaches using biomaterials to host insulin-producing cells derived from stem cells have been developed to overcome the limitations of type 1 diabetes treatment (exogenous insulin injection). However, none seem to fulfill all requirements needed to induce pancreatic cells successful colonization of the scaffolds. Here, we report a polymeric platform adherent to the native mice pancreas filled with human adipose stem cells (hASCs) that was able to induce growth of pancreatic parenchyma. MAIN METHODS: Synthetic polyether-polyurethane discs were placed adjacent to pancreas of normoglycemic and streptozotocin-induced diabetic mice. At day 4 post implantation, 1×106 hASCs were injected intra-implant in groups of normoglycemic and diabetic mice. Immunohistochemistry analysis of the implants was performed to identify insulin positive cells in the newly formed tissue. In addition, metabolic, inflammatory and angiogenic parameters were carried out in those mice. KEY FINDINGS: This study provides evidence of the ability of a biohybrid device to induce the growth of differentiated pancreas parenchyma in both normoglycemic and streptozotocin-induced diabetic mice as detected by histological analysis. Glucose metabolism and body weight of hyperglycemic mice bearing hASCs implants improved. SIGNIFICANCE: The synthetic porous scaffold bearing hASC cells placed adjacent to the native animal pancreas exhibits the potential to be exploited in future cell-based type 1 diabetes therapies.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polyurethanes / Regeneration / Stem Cells / Adipose Tissue / Stem Cell Transplantation / Diabetes Mellitus, Experimental / Insulin-Secreting Cells / Extracellular Matrix Type of study: Prognostic_studies Limits: Animals / Humans / Male Language: En Journal: Life Sci Year: 2017 Document type: Article Affiliation country: Brazil Country of publication: Netherlands

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Polyurethanes / Regeneration / Stem Cells / Adipose Tissue / Stem Cell Transplantation / Diabetes Mellitus, Experimental / Insulin-Secreting Cells / Extracellular Matrix Type of study: Prognostic_studies Limits: Animals / Humans / Male Language: En Journal: Life Sci Year: 2017 Document type: Article Affiliation country: Brazil Country of publication: Netherlands