Actinomyces liubingyangii sp. nov. isolated from the vulture Gypaetus barbatus.
Int J Syst Evol Microbiol
; 67(6): 1873-1879, 2017 Jun.
Article
in En
| MEDLINE
| ID: mdl-28629506
Two strains (VUL4_1T and VUL4_2) of Gram-staining-positive, catalase-negative, non-spore-forming short rods were isolated from rectal swabs of Old World vultures (Gypaetus barbatus) in the Tibet-Qinghai Plateau, China. Analysis of morphological characteristics and biochemical tests indicated that the two strains closely resembled each other but were distinct from other species of the genus Actinomyces previously described. Based on the results of 16S rRNA gene sequence comparison and genome analysis, strains were determined to be members of the genus Actinomyces, closely related to the type strains of Actinomyces marimammalium (96.4â% 16S rRNA gene sequence similarity), Actinomyceshongkongensis (92.4â%), Actinomyceshordeovulneris (92.3â%) and Actinomycesnasicola (92.2â%), respectively. Optimal growth conditions were 37 °C, pH 6-7, with 1â% (w/v) NaCl. Strain VUL4_1T contained C18â:â1ω9c and C16â:â0 as the major cellular fatty acids and diphosphatidylglycerol as the major component of the polar lipids. The genomic DNA G+C content of VUL4_1T was 54.9 mol%. Strain VUL4_1T showed less than 70â% DNA-DNA relatedness with other species of the genus Actinomyces, further supporting strain VUL4_1T as a representative of a novel species. Based on the phenotypic data and phylogenetic inference, a novel species, Actinomyces liubingyangii sp. nov., is proposed with VUL4_1T (=CGMCC 4.7370T=DSM 104050T) as the type strain.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phylogeny
/
Rectum
/
Falconiformes
Limits:
Animals
Country/Region as subject:
Asia
Language:
En
Journal:
Int J Syst Evol Microbiol
Journal subject:
MICROBIOLOGIA
Year:
2017
Document type:
Article
Country of publication:
United kingdom