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Study of infectious diseases in archaeological bone material - A dataset.
Pucu, Elisa; Cascardo, Paula; Chame, Marcia; Felice, Gisele; Guidon, Niéde; Cleonice Vergne, Maria; Campos, Guadalupe; Roberto Machado-Silva, José; Leles, Daniela.
Affiliation
  • Pucu E; Laboratório de Biologia Molecular de Parasitos e de Paleoparasitologia, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Rua Professor Hernani Melo, n. 101, Bairro São Domingos, Niterói, Rio de Janeiro, Brazil.
  • Cascardo P; Laboratório de Biologia Molecular de Parasitos e de Paleoparasitologia, Departamento de Microbiologia e Parasitologia, Instituto Biomédico, Universidade Federal Fluminense, Rua Professor Hernani Melo, n. 101, Bairro São Domingos, Niterói, Rio de Janeiro, Brazil.
  • Chame M; Laboratório de Paleoparasitologia, Escola Nacional de Saúde Pública, Fiocruz, Rio de Janeiro, Rio de Janeiro, Brazil.
  • Felice G; Fundação Museu do Homem Americano, São Raimundo Nonato, Piauí, Brazil.
  • Guidon N; Universidade Federal do Vale do São Francisco, Campus Serra de Capivara, São Raimundo Nonato, Piauí, Brazil.
  • Cleonice Vergne M; Fundação Museu do Homem Americano, São Raimundo Nonato, Piauí, Brazil.
  • Campos G; Universidade do Estado da Bahia, Salvador, Brazil.
  • Roberto Machado-Silva J; Museu de Astronomia e Ciências Afins, Rio de Janeiro, Rio de Janeiro, Brazil.
  • Leles D; Universidade do Estado do Rio de Janeiro, Brazil.
Data Brief ; 13: 692-695, 2017 Aug.
Article in En | MEDLINE | ID: mdl-28725674
Bones of human and ground sloth remains were analyzed for presence of Trypanosoma cruzi by conventional PCR using primers TC, TC1 and TC2. Sequence results amplified a fragment with the same product size as the primers (300 and 350pb). Amplified PCR product was sequenced and analyzed on GenBank, using Blast. Although these sequences did not match with these parasites they showed high amplification with species of bacteria. This article presents the methodology used and the alignment of the sequences. The display of this dataset will allow further analysis of our results and discussion presented in the manuscript "Finding the unexpected: a critical view on molecular diagnosis of infectious diseases in archaeological samples" (Pucu et al. 2017) [1].
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Data Brief Year: 2017 Document type: Article Affiliation country: Brazil Country of publication: Netherlands

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Data Brief Year: 2017 Document type: Article Affiliation country: Brazil Country of publication: Netherlands