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Evaluation of OMNIgene®â€¢SPUTUM for tuberculosis sample processing and liquid culture at a hospital laboratory in India.
Nambiar, Remya; Shaw, Kaitlyn; Curry, Patricia S.
Affiliation
  • Nambiar R; Microbiology Section, Department of Laboratory Medicine, PD Hinduja Hospital and Medical Research Centre, Floor 5, Lalita Giridhar (S1) Building, Off Veer Savarkar Marg, Mahim (West), Mumbai 400016, India.
  • Shaw K; DNA Genotek, 3000 - 500 Palladium Drive, Ottawa, Ontario K2V 1C2, Canada. Electronic address: kaitlyn.shaw@dnagenotek.com.
  • Curry PS; DNA Genotek, 3000 - 500 Palladium Drive, Ottawa, Ontario K2V 1C2, Canada. Electronic address: pat.curry@dnagenotek.com.
J Microbiol Methods ; 141: 60-62, 2017 10.
Article in En | MEDLINE | ID: mdl-28754448
This preliminary evaluation examined the reagent OMNIgene®â€¢SPUTUM (OM-S) as a tool to eliminate NaOH/NALC processing prior to Middlebrook liquid culture for Mycobacterium tuberculosis (MTb). Twenty-seven manually split samples (OM-S-treated vs. NaOH/NALC) showed 100% agreement: 81.5% MTb-positive and 18.5% MTb-negative. On average, OM-S-treated specimens required 1.2 additional days to culture positivity.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Specimen Handling / Sputum / Tuberculosis, Pulmonary / Bacteriological Techniques / Mycobacterium tuberculosis Type of study: Diagnostic_studies / Evaluation_studies Limits: Humans Country/Region as subject: Asia Language: En Journal: J Microbiol Methods Year: 2017 Document type: Article Affiliation country: India Country of publication: Netherlands

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Specimen Handling / Sputum / Tuberculosis, Pulmonary / Bacteriological Techniques / Mycobacterium tuberculosis Type of study: Diagnostic_studies / Evaluation_studies Limits: Humans Country/Region as subject: Asia Language: En Journal: J Microbiol Methods Year: 2017 Document type: Article Affiliation country: India Country of publication: Netherlands